The CBSA's interactomics platform includes an SPR (Surface Plasmon Resonance) apparatus, base molecular docking tools and an MST (MicroScaleThermophoresis) apparatus. This platform is part of the Evreux Platform (PS2E).

The SPR technique is used to visualise interactions between a target fixed on a support and a ligand in the liquid phase, which is injected into the SPRi device, allowing live visualisation of the binding.

The MST approach is used to measure the affinity constants between a labelled target at a constant concentration in 16 capillaries and a potential ligand at 16 different concentrations after a one-half dilution cascade. After checking and validating the amount of fluorescence contained in the 16 capillaries, the 16 thermophoresis curves were completed in 14 minutes. This technique, which requires very little biological product, has enabled the CBSA laboratory to validate several Kd for interactions between peptides and proteins and between protein and RNA.

CBSA laboratory publication references on this topic:

• Rosay et al., 2015 MBio 6(4):e01033-15
• Louis et al., 2022, Adv. Sci. 9(7):e2103262. doi: 10.1002/advs.202103262
• Soussan et al., 2023 Front. Microbiol. 13:1070116
• Salze et al., 2020, RNA Biol Jun; 17(6):794-804
• Karami et al., 2023, J. Chem. Inf. Model. doi: 10.1021/acs.jcim.3c00865.

Contacts: Florian Defontaine: florian.defontaine1@univ-rouen.fr or Olvier Lesouhaitier: olivier.lesouhaitier@univ-rouen.fr